The 3 Rs of Cdc6: Recruitment, Regulation, and Replication
نویسنده
چکیده
What is it? The cell division cycle genes CDC6 and CDC18 encode budding and fission yeast orthologs identified in a series of elegant screens for regulators of cell cycle progression performed independently by Leland Hartwell and Paul Nurse in the 1970s. In the subsequent three decades, orthologs have been found throughout Eukaryota and have been revealed to play key roles in two fundamental aspects of replication, recruiting replication components to chromosomes, and helping safeguard against multiple rounds of DNA replication.
منابع مشابه
The regulation of competence to replicate in meiosis by Cdc6 is conserved during evolution.
DNA replication licensing is an important step in the cell cycle at which cells become competent for DNA replication. When the cell cycle is arrested for long periods of time, this competence is lost. This is the case for somatic cells arrested in G0 or vertebrate oocytes arrested in G2. CDC6 is a factor involved in replication initiation competence which is necessary for the recruitment of the...
متن کاملThe Replication Protein Cdc6 Suppresses Centrosome Over-Duplication in a Manner Independent of Its ATPase Activity
The Cdc6 protein is essential for the initiation of chromosomal replication and functions as a licensing factor to maintain chromosome integrity. During the S and G2 phases of the cell cycle, Cdc6 has been found to inhibit the recruitment of pericentriolar material (PCM) proteins to the centrosome and to suppress centrosome over-duplication. In this report, we analyzed the correlation between t...
متن کاملRecruitment of ORC or CDC6 to DNA is sufficient to create an artificial origin of replication in mammalian cells.
Origins of replication are expected to recruit initiation proteins like origin recognition complex (ORC) and Cdc6 in eukaryotes and provide a platform for unwinding DNA. Here we test whether localization of initiation proteins onto DNA is sufficient for origin function. Different components of the ORC complex and Cdc6 stimulated prereplicative complex (pre-RC) formation and replication initiati...
متن کاملCdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication
To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that although Cdc6 ATP hydrolysis is essential to initiate DNA replication, it is not esse...
متن کاملRedundant and differential regulation of multiple licensing factors ensures prevention of re-replication in normal human cells.
When human cells enter S-phase, overlapping differential inhibitory mechanisms downregulate the replication licensing factors ORC1, CDC6 and Cdt1. Such regulation prevents re-replication so that deregulation of any individual factor alone would not be expected to induce overt re-replication. However, this has been challenged by the fact that overexpression of Cdt1 or Cdt1+CDC6 causes re-replica...
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عنوان ژورنال:
- Current Biology
دوره 12 شماره
صفحات -
تاریخ انتشار 2002